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2021 High quality Hifi Sequencing Provider - Eukaryotic mRNA sequencing – Illumina – Biomarker

Service Details

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Our advancement depends over the highly developed devices, excellent talents and continually strengthened technology forces for Mirna Sequencing Pricing , Pacbio Sequel Ii , Dna Sequencing Service , Because we stay in this line about 10 years. We got best suppliers support on quality and price. And we had weed out suppliers with poor quality. Now many OEM factories cooperated with us too.

2021 High quality Hifi Sequencing Provider - Eukaryotic mRNA sequencing – Illumina – Biomarker Detail:

Advantages

Sample Requirements and Delivery

Library	Sequencing strategy	Data recommended	Quality Control
Poly A enriched	Illumina PE150	6 Gb	Q30≥85%

Sample Requirements:

Nucleotides:

Purity	Integrity	Amount
OD260/280≥1.7-2.5 OD260/230≥0.5-2.5Limited or no protein or DNA contamination shown on gel.	For plants: RIN≥6.5;For animals: RIN≥7;28S/18S≥1.0;limited or no baseline elevation	Conc. ≥30 ng/μl; Volumn ≥ 10 μl; Total ≥ 1.5 μg

Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.

Cell suspension: Cell count = 3×10⁶ - 1×10⁷
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended, which is preferable for micro extraction.

Blood samples: Volume≥1 ml

Microorganism: Mass ≥ 1 g

Recommended Sample Delivery

Container: 2 ml centrifuge tube (Tin foil is not recommended)

Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …

Shipment:

Dry-ice: Samples need to be packed in bags and buried in dry-ice.
RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.

Service Work Flow

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Experiment design

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Sample delivery

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RNA extraction

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Library construction

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Sequencing

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Data analysis

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After-sale services

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"Sincerity, Innovation, Rigorousness, and Efficiency" is the persistent conception of our company for the long-term to develop together with customers for mutual reciprocity and mutual benefit for 2021 High quality Hifi Sequencing Provider - Eukaryotic mRNA sequencing – Illumina – Biomarker , The product will supply to all over the world, such as: Tunisia , Malaysia , Lithuania , Since its foundation , the company keeps living up to the belief of "honest selling , best quality , people-orientation and benefits to customers. " We are doing everything to offer our customers with best services and best products . We promise that we'll be responsible all the way to the end once our services begin.

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This enterprise in the industry is strong and competitive, advancing with the times and develop sustainable, we are very pleased to have a opportunity to cooperate!