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Genbank
, Bioinformatics Data Analysis
, Operational Taxonomic Unit
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Nanopore-based metagenome sequencing – Biomarker Detail:
Service Advantages
Service Specifications
Bioinformatics analyses
ü Raw data quality control
ü Metagenome assembly
ü Non-redundant gene set and annotation
ü Species diversity analysis
ü Genetic function diversity analysis
ü Inter-group analysis
ü Association analysis against experimental factors
Sample Requirements and Delivery
Sample requirements and delivery
Sample Requirements:
For DNA extracts:
Sample Type
|
Amount
|
Concentration
|
Purity
|
DNA extracts
|
> 30 ng
|
> 1 ng/μl
|
OD260/280= 1.6-2.5
|
For environmental samples:
Sample type
|
Recommended sampling procedure |
Soil
|
Sampling amount: approx. 5 g; Remaining withered substance needs to be removed from surface; Grind large pieces and pass through 2 mm filter; Aliquot samples in sterile EP-tube or cyrotube for reservation. |
Feces
|
Sampling amount: approx. 5 g; Collect and aliquot samples in sterile EP-tube or cryotube for reservation. |
Intestinal contents
|
Samples need to be processed under aseptic condition. Wash collected tissue with PBS; Centrifuge the PBS and collect the precipitant in EP-tubes. |
Sludge
|
Sampling amount: approx. 5 g; Collect and aliquot sludge sample in sterile EP-tube or cryotube for reservation |
Waterbody
|
For sample with limited amount of microbial, such as tap water, well water, etc., Collect at least 1 L water and pass through 0.22 μm filter to enrich microbial on the membrane. Store the membrane in sterile tube. |
Skin
|
Carefully scrape skin surface with sterile cotton swab or surgical blade and place it in sterile tube. |
Recommended Sample Delivery
Freeze the samples in liquid nitrogen for 3-4 hours and store in liquid nitrogen or -80 degree to long-term reservation. Sample shipping with dry-ice is required.
Service Work Flow
Sample delivery
Library construction
Sequencing
Data analysis
After-sale services
Product detail pictures:
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Nanopore-based metagenome sequencing – Biomarker , The product will supply to all over the world, such as: Russia
, Argentina
, Germany
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