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2021 Good Quality Ngs Workflow - Nanopore-based metagenome sequencing – Biomarker

Service Details

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2021 Good Quality Ngs Workflow - Nanopore-based metagenome sequencing – Biomarker Detail:

Service Advantages

Service Specifications

Bioinformatics analyses

ü Raw data quality control

ü Metagenome assembly

ü Non-redundant gene set and annotation

ü Species diversity analysis

ü Genetic function diversity analysis

ü Inter-group analysis

ü Association analysis against experimental factors

Sample Requirements and Delivery

Sample requirements and delivery

Sample Requirements:

For DNA extracts:

Sample Type	Amount	Concentration	Purity
DNA extracts	＞ 30 ng	＞ 1 ng/μl	OD260/280= 1.6-2.5

For environmental samples:

Sample type	Recommended sampling procedure
Soil	Sampling amount: approx. 5 g; Remaining withered substance needs to be removed from surface; Grind large pieces and pass through 2 mm filter; Aliquot samples in sterile EP-tube or cyrotube for reservation.
Feces	Sampling amount: approx. 5 g; Collect and aliquot samples in sterile EP-tube or cryotube for reservation.
Intestinal contents	Samples need to be processed under aseptic condition. Wash collected tissue with PBS; Centrifuge the PBS and collect the precipitant in EP-tubes.
Sludge	Sampling amount: approx. 5 g; Collect and aliquot sludge sample in sterile EP-tube or cryotube for reservation
Waterbody	For sample with limited amount of microbial, such as tap water, well water, etc., Collect at least 1 L water and pass through 0.22 μm filter to enrich microbial on the membrane. Store the membrane in sterile tube.
Skin	Carefully scrape skin surface with sterile cotton swab or surgical blade and place it in sterile tube.

Recommended Sample Delivery

Freeze the samples in liquid nitrogen for 3-4 hours and store in liquid nitrogen or -80 degree to long-term reservation. Sample shipping with dry-ice is required.

Service Work Flow

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Sample delivery

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Library construction

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Sequencing

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Data analysis

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After-sale services

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It adheres on the tenet "Honest, industrious, enterprising, innovative" to develop new items frequently. It regards buyers, success as its very own success. Let us produce prosperous future hand in hand for 2021 Good Quality Ngs Workflow - Nanopore-based metagenome sequencing – Biomarker , The product will supply to all over the world, such as: Japan , Czech Republic , Cairo , Immediate and specialist after-sale service supplied by our consultant group has happy our buyers. Detailed Info and parameters from the merchandise will probably be sent to you for any thorough acknowledge. Free samples may be delivered and company check out to our corporation. n Morocco for negotiation is constantly welcome. Hope to get inquiries type you and construct a long-term co-operation partnership.

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Managers are visionary, they have the idea of "mutual benefits, continuous improvement and innovation", we have a pleasant conversation and Cooperation.

By Kimberley from Latvia - 2017.03.08 14:45

This is the first business after our company establish, products and services are very satisfying, we have a good start, we hope to cooperate continuous in the future!

By jari dedenroth from Islamabad - 2018.07.12 12:19