100% Original Factory Oxford Nanopore Provider - Eukaryotic mRNA sequencing – Illumina – Biomarker
100% Original Factory Oxford Nanopore Provider - Eukaryotic mRNA sequencing – Illumina – Biomarker Detail:
Advantages
Sample Requirements and Delivery
| Library | Sequencing strategy | Data recommended | Quality Control |
| Poly A enriched | Illumina PE150 |
6 Gb |
Q30≥85% |
Sample Requirements:
Nucleotides:
| Purity | Integrity | Amount |
| OD260/280≥1.7-2.5 OD260/230≥0.5-2.5Limited or no protein or DNA contamination shown on gel. | For plants: RIN≥6.5;For animals: RIN≥7;28S/18S≥1.0;limited or no baseline elevation | Conc. ≥30 ng/μl; Volumn ≥ 10 μl; Total ≥ 1.5 μg |
Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.
Cell suspension: Cell count = 3×106 - 1×107
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended, which is preferable for micro extraction.
Blood samples: Volume≥1 ml
Microorganism: Mass ≥ 1 g
Recommended Sample Delivery
Container: 2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …
Shipment:
- Dry-ice: Samples need to be packed in bags and buried in dry-ice.
- RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.
Service Work Flow
Experiment design
Sample delivery
RNA extraction
Library construction
Sequencing
Data analysis
After-sale services
Product detail pictures:
Related Product Guide:
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By Dolores
from America
- 2017.08.18 11:04
The company keeps to the operation concept "scientific management, high quality and efficiency primacy, customer supreme", we have always maintained business cooperation. Work with you,we feel easy!
By Amy
from Curacao
- 2017.09.28 18:29
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